| 品牌: 美国NewEast Biosciences |
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| 货号: 26200 |
| 抗原: Syntheticpeptide |
| 规格: 100微升 |
| 产品全名: Anti-BRAF(D594G)Mouse Monoclonal Antibody |
| 应用: ELISA,WB |
| 种属反应性的选择: Human,Mouse and Rat |
| 物种/宿主: Mouse |
| 货号: 26200 |
| 基因符号: BRAF, BRAF1, RAFB1 |
| 描述: Anti-BRAF(D594G) Mouse Monoclonal Antibody |
| 背景介绍: BRAF belongs to a family of serine-threonineprotein kinases. As a part of a signaling pathway known as the theRAS/MAPK pathway, it plays an important part in many cellularprocesses. The processes include cell proliferation,differentiation and transcriptional regulation. Mutations in theBRAF gene cause diseases. Inherited mutations in BRAF causecardiofaciocutaneous syndrome. Acquired mutations in BRAF have beenfound in cancers. |
| 免疫原: A synthetic peptide from the internal region ofBRAF, which includes the mutation of D594G, human origin |
| 经过测试的应用: ELISA, WB |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 1000 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS(without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes D594G mutant, but not wild-type BRAFof vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezingand thawing |
蛋白质印迹:  Westernblot analysis of recombinant BRAF(D594G) and wild typeproteins. Purified His-tagged BRAF(D594G) protein (lane2) and corresponding wild type protein (lane 1) were blotted withAnti-BRAF(D594G) monoclonal antibody (Cat. # 26200). |